Regulatory

Part:BBa_K3927001:Design

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-09-29)


C120-CYC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 86
    Illegal SpeI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 104
    Illegal SpeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion signal peptide is required for the yeast chassis to secrete the peptide.


Source

Gene sequence is taken from NCBI

References